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KMID : 0613820080180070918
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Journal of Life Science
2008 Volume.18 No. 7 p.918 ~ p.923
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Identification of Phosphoproteins Induced by AT1 Receptor Blocker Losartan
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Lee Chang-Woo
Kim Mi-jin
Jang Sei-Heon
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Abstract
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The angiotensin Ⅱ receptor (AT₁R) antagonists are effective in treating patients with hypertension and showed beneficial effects in diabetes and other metabolic diseases. The beneficial effects of AT₁R antagonists are mainly considered to be from inhibition of Ang Ⅱ-AT₁R signaling pathway such as the activation of NADPH oxidase and the generation of reactive oxygen species. In this study, we examined whether antagonist of the AT1R could account for phosphorylation of proteins in cells using antibody array. We have selected 6 proteins with Ser/Thr-phosphorylation sites and 12 proteins with Tyr-phosphorylation sites based on literature search. Upon AT1R antagonist losartan treatment to serum-starved COS-1 cells, there was ～20% increase of Ser phosphorylation in small GTPase RhoA. RhoA is known to be responsible for cytoskeleton rearrangement and is down-regulated upon Ser phosphorylation in vivo. Our finding provides a new insight into the mechanism and signaling pathway of the AT1R antagonist in cells.
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KEYWORD
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AngiotensinⅡ(AT1)receptor, antibodyarray, phosphorylation, RhoA
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